An Aluminum Marker for the Differentiation and Separation of Virulent and Attenuated Polioviruses

10 VIRULENT AND ATTENUATED POLIOVIRUSES Attenuated

This paper is concerned with the correlation of an in ~tro marker of poliovirus particles, the capacity for multiplication on a monkey stable (MS) cell line, with thc degree of ncurovirulence for monkeys. Vogt a M. (I) discovered that the plaque formation of most attenuated strains of poliovirus is delaycd or inhibited, when the amount of bicarbonate in the agar overlay is reduced. However, a n...

Determination of CD Markers Profile of the Cell Line Infected by S15 Vaccine Strain of Theileria annulata Schizont Using RT-PCR Analysis

The aim of this study was to identify the cell surface cluster of differentiation (CD) markers of the cell lines infected by Theileria annulata schizont. The CD molecules are very useful for the characterization of cells and different subpopulations of leukocytes. They are usually recognized by specific antibodies using flow cytometry and immunohistochemistry. In the current stud...

Role of Interferon in Replication of Virulent and Attenuated Strains of Measles Virus

Neurovirulence of type 1 polioviruses isolated from sewage in Japan.

Sixteen type 1 poliovirus strains were isolated from a sewage disposal plant located downstream of the Oyabe River in Japan between October 1993 and September 1995. The isolates were intratypically differentiated as vaccine-derived strains. Neutralizing antigenicity analysis with monoclonal antibodies and estimation of neurovirulence by mutant analysis by PCR and restriction enzyme cleavage (MA...

Next Generation Inactivated Polio Vaccine Manufacturing to Support Post Polio-Eradication Biosafety Goals

Worldwide efforts to eradicate polio caused a tipping point in polio vaccination strategies. A switch from the oral polio vaccine, which can cause circulating and virulent vaccine derived polioviruses, to inactivated polio vaccines (IPV) is scheduled. Moreover, a manufacturing process, using attenuated virus strains instead of wild-type polioviruses, is demanded to enhance worldwide production ...